Terminator

Part:BBa_K3726095

Designed by: Lucia Tapia   Group: iGEM21_MADRID_UCM   (2021-10-06)


T(5a)_Teck

The sequence of this parts corresponds with a rho-independent (intrinsec) terminator with a proved 99.9% terminating efficiency in UTEX 2973 either in optimal and suboptimal growth conditions.

The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.


Sequence & Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None